James Dahlman, PhD

Research Program

Discovery and Developmental Therapeutics
Phone Number

404-385-5262
Email Address

james.dahlman@bme.gatech.edu

Biography

James Dahlman, PhD, is an associate professor and holds the McCamish Foundation Early Career Professorship in the Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech University. Dr. Dahlman is a member of the Discovery and Developmental Therapeutics Research Program at Winship Cancer Institute of Emory University in Atlanta, Georgia.

Education

Dr. Dahlman received his PhD from MIT and Harvard Medical School in 2014, where he studied RNA delivery with Robert Langer and Daniel Anderson. He studied RNA design and gene editing as a post-doc with Feng Zhang at The Broad Institute.

Titles & Roles

Associate Professor, McCamish Foundation Early Career Professorship, Department of Biomedical Engineering

Georgia Institute of Technology

Research

Dr. Dahlman is interested in drug delivery, targeted in vivo gene editing and using genomics to improve biomaterial design. He has designed and synthesized nanoparticles that efficiently deliver RNAs to the lung and heart. These nanoparticles can deliver multiple RNAs at once, and can simultaneously knockdown five genes concurrently in vivo. They have been used by over 10 labs across the United States to study cancer, atherosclerosis, inflammation, emphysema and pulmonary hypertension, and are being evaluated for clinical trials.

Publications

Title/Journal	Authors	Publication Date
Nanoparticle delivery of a prodrug-activating bacterial enzyme leads to anti-tumor responses. Nature communications	SG Huayamares, L Lian, R Rab, Y Hou, A Radmand, H Kim, R Zenhausern, BR Achyut, M Gilbert Ross, MP Lokugamage, D Loughrey, HE Peck, ES Echeverri, AJ Da Silva Sanchez, A Shajii, A Li, KE Tiegreen, PJ Santangelo, EJ Sorscher, JE Dahlman	04/12/2025
The time course of in vivo cellular responses to LNPs. Chemical communications (Cambridge, England)	D Loughrey, K Paunovska, ES Echeverri, KE Tiegreen, JE Dahlman	03/13/2025
Amelioration of a von Willebrand disease type 2B phenotype in vivo upon treatment with allele-selective siRNAs. Blood advances	NA Linthorst, YK Jongejan, RJ Dirven, SNJ Laan, R Bierings, C Casari, RA Cordfunke, JE Dahlman, N Dolezal, JW Drijfhout, FWG Leebeek, LR Ruhaak, E Schrader Echeverri, J Voorberg, BJM van Vlijmen, CV Denis, JCJ Eikenboom	01/28/2025
Silencing of the von Willebrand factor gene in proatherothrombotic APOE∗3-Leiden.CETP transgenic mice. Research and practice in thrombosis and haemostasis	YK Jongejan, RJ Dirven, E Schrader Echeverri, AJL de Jong, ACM Pronk, S Kooijman, PCN Rensen, JE Dahlman, JCJ Eikenboom, BJM van Vlijmen	01/01/2025
Lipid nanoparticle-mediated mRNA delivery to CD34⁺ cells in rhesus monkeys. Nature biotechnology	H Kim, R Zenhausern, K Gentry, L Lian, SG Huayamares, A Radmand, D Loughrey, AR Podilapu, MZC Hatit, H Ni, A Li, A Shajii, HE Peck, K Han, X Hua, S Jia, M Martinez, C Lee, PJ Santangelo, A Tarantal, JE Dahlman	11/22/2024
Lymphatic endothelial cell-targeting lipid nanoparticles delivering VEGFC mRNA improve lymphatic function after injury. bioRxiv : the preprint server for biology	E Michalaki, R Chin, K Jeong, Z Qi, LN Liebman, Y González-Vargas, ES Echeverri, K Paunovska, H Muramatsu, N Pardi, BJ Tamburini, Z Jakus, JE Dahlman, JB Dixon	07/31/2024
Substituting Poly(ethylene glycol) Lipids with Poly(2-ethyl-2-oxazoline) Lipids Improves Lipid Nanoparticle Repeat Dosing. Advanced healthcare materials	AJDS Sanchez, D Loughrey, ES Echeverri, SG Huayamares, A Radmand, K Paunovska, M Hatit, KE Tiegreen, PJ Santangelo, JE Dahlman	07/01/2024
Nucleic acid-based drugs for patients with solid tumours. Nature reviews. Clinical oncology	SG Huayamares, D Loughrey, H Kim, JE Dahlman, EJ Sorscher	06/01/2024
Impact of allele-selective silencing of von Willebrand factor in mice based on a single nucleotide allelic difference in von Willebrand factor. Thrombosis research	YK Jongejan, NA Linthorst, E Schrader Echeverri, SNJ Laan, RJ Dirven, JE Dahlman, BJM van Vlijmen, CV Denis, JCJ Eikenboom	04/01/2024
Cationic cholesterol-dependent LNP delivery to lung stem cells, the liver, and heart. Proceedings of the National Academy of Sciences of the United States of America	A Radmand, H Kim, J Beyersdorf, CN Dobrowolski, R Zenhausern, K Paunovska, SG Huayamares, X Hua, K Han, D Loughrey, MZC Hatit, A Del Cid, H Ni, A Shajii, A Li, A Muralidharan, HE Peck, KE Tiegreen, S Jia, PJ Santangelo, JE Dahlman	03/12/2024
RNA delivery systems. Proceedings of the National Academy of Sciences of the United States of America	SN Bhatia, JE Dahlman	03/12/2024
Light-field flow cytometry for high-resolution, volumetric and multiparametric 3D single-cell analysis. Nature communications	X Hua, K Han, B Mandracchia, A Radmand, W Liu, H Kim, Z Yuan, SM Ehrlich, K Li, C Zheng, J Son, AD Silva Trenkle, GA Kwong, C Zhu, JE Dahlman, S Jia	03/04/2024
Multivalent Targeting of the Asialoglycoprotein Receptor by Virus-Like Particles. Small (Weinheim an der Bergstrasse, Germany)	R Hincapie, S Bhattacharya, MM Baksh, CA Sanhueza, ES Echeverri, H Kim, K Paunovska, AR Podilapu, M Xu, JE Dahlman, MG Finn	12/01/2023
Drug delivery systems for CRISPR-based genome editors. Nature reviews. Drug discovery	V Madigan, F Zhang, JE Dahlman	11/01/2023
Small interfering RNA-mediated allele-selective silencing of von Willebrand factor in vitro and in vivo. Blood advances	YK Jongejan, E Schrader Echeverri, RJ Dirven, K Paunovska, NA Linthorst, A de Jong, JC Wellershoff, KD van der Gouw, BJM van Vlijmen, JE Dahlman, JCJ Eikenboom	10/24/2023
Nanoparticle stereochemistry-dependent endocytic processing improves in vivo mRNA delivery. Nature chemistry	MZC Hatit, CN Dobrowolski, MP Lokugamage, D Loughrey, H Ni, C Zurla, AJ Da Silva Sanchez, A Radmand, SG Huayamares, R Zenhausern, K Paunovska, HE Peck, J Kim, M Sato, JI Feldman, MA Rivera, A Cristian, Y Kim, PJ Santangelo, JE Dahlman	04/01/2023
The Transcriptional Response to Lung-Targeting Lipid Nanoparticles in Vivo. Nano letters	A Radmand, MP Lokugamage, H Kim, C Dobrowolski, R Zenhausern, D Loughrey, SG Huayamares, MZC Hatit, H Ni, A Del Cid, AJ Da Silva Sanchez, K Paunovska, E Schrader Echeverri, A Shajii, H Peck, PJ Santangelo, JE Dahlman	02/08/2023
Current challenges and future directions for engineering extracellular vesicles for heart, lung, blood and sleep diseases. Journal of extracellular vesicles	G Li, T Chen, J Dahlman, L Eniola-Adefeso, IC Ghiran, P Kurre, WA Lam, JK Lang, E Marbán, P Martín, S Momma, M Moos, DJ Nelson, RL Raffai, X Ren, JPG Sluijter, SL Stott, G Vunjak-Novakovic, ND Walker, Z Wang, KW Witwer, PC Yang, MS Lundberg, MJ Ochocinska, R Wong, G Zhou, SY Chan, S Das, P Sundd	02/01/2023
Substituting racemic ionizable lipids with stereopure ionizable lipids can increase mRNA delivery. Journal of controlled release : official journal of the Controlled Release Society	AJ Da Silva Sanchez, K Zhao, SG Huayamares, MZC Hatit, MP Lokugamage, D Loughrey, C Dobrowolski, S Wang, H Kim, K Paunovska, Y Kuzminich, JE Dahlman	01/01/2023
The Extent to Which Lipid Nanoparticles Require Apolipoprotein E and Low-Density Lipoprotein Receptor for Delivery Changes with Ionizable Lipid Structure. Nano letters	K Paunovska, AJ Da Silva Sanchez, MP Lokugamage, D Loughrey, ES Echeverri, A Cristian, MZC Hatit, PJ Santangelo, K Zhao, JE Dahlman	12/28/2022
What are the current bottlenecks in developing and applying CRISPR technologies? Cell systems	EH Kellogg, J Gootenberg, O Abudayyeh, ASL Wong, JE Dahlman, A Lapinaite, C Myhrvold, CC Liu, PD Hsu, P Mali, LS Qi	08/17/2022
Piperazine-derived lipid nanoparticles deliver mRNA to immune cells in vivo. Nature communications	H Ni, MZC Hatit, K Zhao, D Loughrey, MP Lokugamage, HE Peck, AD Cid, A Muralidharan, Y Kim, PJ Santangelo, JE Dahlman	08/15/2022
Nanoparticle single-cell multiomic readouts reveal that cell heterogeneity influences lipid nanoparticle-mediated messenger RNA delivery. Nature nanotechnology	C Dobrowolski, K Paunovska, E Schrader Echeverri, D Loughrey, AJ Da Silva Sanchez, H Ni, MZC Hatit, MP Lokugamage, Y Kuzminich, HE Peck, PJ Santangelo, JE Dahlman	08/01/2022
Universal Barcoding Predicts In Vivo ApoE-Independent Lipid Nanoparticle Delivery. Nano letters	AJ Da Silva Sanchez, C Dobrowolski, A Cristian, ES Echeverri, K Zhao, MZC Hatit, D Loughrey, K Paunovska, JE Dahlman	06/22/2022
Drug delivery systems for RNA therapeutics. Nature reviews. Genetics	K Paunovska, D Loughrey, JE Dahlman	05/01/2022
Species-dependent in vivo mRNA delivery and cellular responses to nanoparticles. Nature nanotechnology	MZC Hatit, MP Lokugamage, CN Dobrowolski, K Paunovska, H Ni, K Zhao, D Vanover, J Beyersdorf, HE Peck, D Loughrey, M Sato, A Cristian, PJ Santangelo, JE Dahlman	03/01/2022
Augmented lipid-nanoparticle-mediated in vivo genome editing in the lungs and spleen by disrupting Cas9 activity in the liver. Nature biomedical engineering	CD Sago, MP Lokugamage, D Loughrey, KE Lindsay, R Hincapie, BR Krupczak, S Kalathoor, M Sato, ES Echeverri, JP Fitzgerald, Z Gan, L Gamboa, K Paunovska, CA Sanhueza, MZC Hatit, MG Finn, PJ Santangelo, JE Dahlman	02/01/2022
Non-liver mRNA Delivery. Accounts of chemical research	D Loughrey, JE Dahlman	01/04/2022
Optimization of lipid nanoparticles for the delivery of nebulized therapeutic mRNA to the lungs. Nature biomedical engineering	MP Lokugamage, D Vanover, J Beyersdorf, MZC Hatit, L Rotolo, ES Echeverri, HE Peck, H Ni, JK Yoon, Y Kim, PJ Santangelo, JE Dahlman	09/01/2021
Therapeutic RNA Delivery for COVID and Other Diseases. Advanced healthcare materials	C Dobrowolski, K Paunovska, MZC Hatit, MP Lokugamage, JE Dahlman	08/01/2021
Frataxin deficiency promotes endothelial senescence in pulmonary hypertension. The Journal of clinical investigation	MK Culley, J Zhao, YY Tai, Y Tang, D Perk, V Negi, Q Yu, CC Woodcock, A Handen, G Speyer, S Kim, YC Lai, T Satoh, AM Watson, YA Aaraj, J Sembrat, M Rojas, D Goncharov, EA Goncharova, OF Khan, DG Anderson, JE Dahlman, AU Gurkar, R Lafyatis, AU Fayyaz, MM Redfield, MT Gladwin, M Rabinovitch, M Gu, T Bertero, SY Chan	06/01/2021
The NIH Somatic Cell Genome Editing program. Nature	K Saha, EJ Sontheimer, PJ Brooks, MR Dwinell, CA Gersbach, DR Liu, SA Murray, SQ Tsai, RC Wilson, DG Anderson, A Asokan, JF Banfield, KS Bankiewicz, G Bao, JWM Bulte, N Bursac, JM Campbell, DF Carlson, EL Chaikof, ZY Chen, RH Cheng, KJ Clark, DT Curiel, JE Dahlman, BE Deverman, ME Dickinson, JA Doudna, SC Ekker, ME Emborg, G Feng, BS Freedman, DM Gamm, G Gao, IC Ghiran, PM Glazer, S Gong, JD Heaney, JD Hennebold, JT Hinson, A Khvorova, S Kiani, WR Lagor, KS Lam, KW Leong, JE Levine, JA Lewis, CM Lutz, DH Ly, S Maragh, PB McCray, TC McDevitt, O Mirochnitchenko, R Morizane, N Murthy, RS Prather, JA Ronald, S Roy, S Roy, V Sabbisetti, WM Saltzman, PJ Santangelo, DJ Segal, M Shimoyama, MC Skala, AF Tarantal, JC Tilton, GA Truskey, M Vandsburger, JK Watts, KD Wells, SA Wolfe, Q Xu, W Xue, G Yi, J Zhou	04/01/2021
Voices of biotech research. Nature biotechnology	N Annabi, M Baker, A Boettiger, D Chakraborty, Y Chen, KS Corbett, B Correia, J Dahlman, T de Oliveira, A Ertuerk, MF Yanik, E Henaff, M Huch, ID Iliev, T Jacobs, H Junca, A Keung, I Kolodkin-Gal, S Krishnaswamy, M Lancaster, E Macosko, MA Martínez-Núñez, K Miura, J Molloy, AO Cruz, RJ Platt, AD Posey, H Shao, M Simunovic, N Slavov, T Takebe, LH Vandenberghe, RK Varshney, J Wang	03/01/2021
Nanoparticles containing constrained phospholipids deliver mRNA to liver immune cells in vivo without targeting ligands. Bioengineering & translational medicine	Z Gan, MP Lokugamage, MZC Hatit, D Loughrey, K Paunovska, M Sato, A Cristian, JE Dahlman	09/01/2020
Treating Cystic Fibrosis with mRNA and CRISPR. Human gene therapy	A Da Silva Sanchez, K Paunovska, A Cristian, JE Dahlman	09/01/2020
Increased PIP3 activity blocks nanoparticle mRNA delivery. Science advances	K Paunovska, A Da Silva Sanchez, MT Foster, D Loughrey, EL Blanchard, FZ Islam, Z Gan, A Mantalaris, PJ Santangelo, JE Dahlman	07/01/2020
Mild Innate Immune Activation Overrides Efficient Nanoparticle-Mediated RNA Delivery. Advanced materials (Deerfield Beach, Fla.)	MP Lokugamage, Z Gan, C Zurla, J Levin, FZ Islam, S Kalathoor, M Sato, CD Sago, PJ Santangelo, JE Dahlman	01/01/2020
Constrained Nanoparticles Deliver siRNA and sgRNA to T Cells In Vivo without Targeting Ligands. Advanced materials (Deerfield Beach, Fla.)	MP Lokugamage, CD Sago, Z Gan, BR Krupczak, JE Dahlman	10/01/2019
Using Large Datasets to Understand Nanotechnology. Advanced materials (Deerfield Beach, Fla.)	K Paunovska, D Loughrey, CD Sago, R Langer, JE Dahlman	10/01/2019
Cell Subtypes Within the Liver Microenvironment Differentially Interact with Lipid Nanoparticles. Cellular and molecular bioengineering	CD Sago, BR Krupczak, MP Lokugamage, Z Gan, JE Dahlman	10/01/2019
Ligand Conjugated Multimeric siRNAs Enable Enhanced Uptake and Multiplexed Gene Silencing. Nucleic acid therapeutics	JM Brown, JE Dahlman, KK Neuman, CAH Prata, MC Krampert, PM Hadwiger, HP Vornlocher	10/01/2019
Endothelial TGF-β signalling drives vascular inflammation and atherosclerosis. Nature metabolism	PY Chen, L Qin, G Li, Z Wang, JE Dahlman, J Malagon-Lopez, S Gujja, NA Cilfone, KJ Kauffman, L Sun, H Sun, X Zhang, B Aryal, A Canfran-Duque, R Liu, P Kusters, A Sehgal, Y Jiao, DG Anderson, J Gulcher, C Fernandez-Hernando, E Lutgens, MA Schwartz, JS Pober, TW Chittenden, G Tellides, M Simons	09/01/2019
All the World's Data Could Fit in an Egg. Scientific American	J Dahlman	06/01/2019
BOLA (BolA Family Member 3) Deficiency Controls Endothelial Metabolism and Glycine Homeostasis in Pulmonary Hypertension. Circulation	Q Yu, YY Tai, Y Tang, J Zhao, V Negi, MK Culley, J Pilli, W Sun, K Brugger, J Mayr, R Saggar, R Saggar, WD Wallace, DJ Ross, AB Waxman, SG Wendell, SJ Mullett, J Sembrat, M Rojas, OF Khan, JE Dahlman, M Sugahara, N Kagiyama, T Satoh, M Zhang, N Feng, J Gorcsan, SO Vargas, KJ Haley, R Kumar, BB Graham, R Langer, DG Anderson, B Wang, S Shiva, T Bertero, SY Chan	05/07/2019
Nanoparticles Containing Oxidized Cholesterol Deliver mRNA to the Liver Microenvironment at Clinically Relevant Doses. Advanced materials (Deerfield Beach, Fla.)	K Paunovska, AJ Da Silva Sanchez, CD Sago, Z Gan, MP Lokugamage, FZ Islam, S Kalathoor, BR Krupczak, JE Dahlman	04/01/2019
Nanoparticles That Deliver RNA to Bone Marrow Identified by in Vivo Directed Evolution. Journal of the American Chemical Society	CD Sago, MP Lokugamage, FZ Islam, BR Krupczak, M Sato, JE Dahlman	12/12/2018
Modifying a Commonly Expressed Endocytic Receptor Retargets Nanoparticles in Vivo. Nano letters	CD Sago, MP Lokugamage, GN Lando, N Djeddar, NN Shah, C Syed, AV Bryksin, JE Dahlman	12/12/2018
Barcoding chemical modifications into nucleic acids improves drug stability in vivo. Journal of materials chemistry. B	CD Sago, S Kalathoor, JP Fitzgerald, GN Lando, N Djeddar, AV Bryksin, JE Dahlman	11/28/2018
Editing nature: Local roots of global governance. Science (New York, N.Y.)	N Kofler, JP Collins, J Kuzma, E Marris, K Esvelt, MP Nelson, A Newhouse, LJ Rothschild, VS Vigliotti, M Semenov, R Jacobsen, JE Dahlman, S Prince, A Caccone, T Brown, OJ Schmitz	11/02/2018
High-throughput in vivo screen of functional mRNA delivery identifies nanoparticles for endothelial cell gene editing. Proceedings of the National Academy of Sciences of the United States of America	CD Sago, MP Lokugamage, K Paunovska, DA Vanover, CM Monaco, NN Shah, M Gamboa Castro, SE Anderson, TG Rudoltz, GN Lando, P Munnilal Tiwari, JL Kirschman, N Willett, YC Jang, PJ Santangelo, AV Bryksin, JE Dahlman	10/16/2018
Testing thousands of nanoparticles in vivo using DNA barcodes. Current opinion in biomedical engineering	MP Lokugamage, CD Sago, JE Dahlman	09/01/2018
Analyzing 2000 in Vivo Drug Delivery Data Points Reveals Cholesterol Structure Impacts Nanoparticle Delivery. ACS nano	K Paunovska, CJ Gil, MP Lokugamage, CD Sago, M Sato, GN Lando, M Gamboa Castro, AV Bryksin, JE Dahlman	08/28/2018
A Direct Comparison of in Vitro and in Vivo Nucleic Acid Delivery Mediated by Hundreds of Nanoparticles Reveals a Weak Correlation. Nano letters	K Paunovska, CD Sago, CM Monaco, WH Hudson, MG Castro, TG Rudoltz, S Kalathoor, DA Vanover, PJ Santangelo, R Ahmed, AV Bryksin, JE Dahlman	03/14/2018
Inhibiting Integrin α5 Cytoplasmic Domain Signaling Reduces Atherosclerosis and Promotes Arteriogenesis. Journal of the American Heart Association	M Budatha, J Zhang, ZW Zhuang, S Yun, JE Dahlman, DG Anderson, MA Schwartz	01/30/2018
Barcoded nanoparticles for high throughput in vivo discovery of targeted therapeutics. Proceedings of the National Academy of Sciences of the United States of America	JE Dahlman, KJ Kauffman, Y Xing, TE Shaw, FF Mir, CC Dlott, R Langer, DG Anderson, ET Wang	02/21/2017
Interaction between integrin α5 and PDE4D regulates endothelial inflammatory signalling. Nature cell biology	S Yun, M Budatha, JE Dahlman, BG Coon, RT Cameron, R Langer, DG Anderson, G Baillie, MA Schwartz	10/01/2016
Proliferation and Recruitment Contribute to Myocardial Macrophage Expansion in Chronic Heart Failure. Circulation research	HB Sager, M Hulsmans, KJ Lavine, MB Moreira, T Heidt, G Courties, Y Sun, Y Iwamoto, B Tricot, OF Khan, JE Dahlman, A Borodovsky, K Fitzgerald, DG Anderson, R Weissleder, P Libby, FK Swirski, M Nahrendorf	09/16/2016
RNAi targeting multiple cell adhesion molecules reduces immune cell recruitment and vascular inflammation after myocardial infarction. Science translational medicine	HB Sager, P Dutta, JE Dahlman, M Hulsmans, G Courties, Y Sun, T Heidt, C Vinegoni, A Borodovsky, K Fitzgerald, GR Wojtkiewicz, Y Iwamoto, B Tricot, OF Khan, KJ Kauffman, Y Xing, TE Shaw, P Libby, R Langer, R Weissleder, FK Swirski, DG Anderson, M Nahrendorf	06/08/2016
Corrigendum: Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Nature biotechnology	JE Dahlman, OO Abudayyeh, J Joung, JS Gootenberg, F Zhang, S Konermann	04/01/2016